Fig 5. Jujuboside B activated eNOS depending on extracellular Ca2+ and TRPC channels.
(A)EGTA and SKF96365 significantly inhibited Jujuboside B-induced eNOS activation. ** P < 0.01 compared with Control, # P < 0.05, ## P <0.01 compared with Jujuboside B, n = 4. (B) Western blot analysis showed that EGTA and SKF96365 significantly inhibited Jujuboside B-induced eNOS phosphorylation at Serine-1177. (C) Densitometric analysis showed that EGTA and SKF96365 significantly inhibited Jujuboside B-induced eNOS phosphorylation at Serine-1177. # P < 0.05 compared with Control, * P < 0.05 compared with Jujuboside B, n = 3.