Fig 8. Jujuboside B increased Ca²⁺ influx through TRPC channels.

(A) Representative confocal image showed that depleting intracellular Ca²⁺ store by thapsigargin initiated a slow and continuous Ca²⁺ influx. (B) Representative confocal image showed that after depleting intracellular Ca²⁺ store by thapsigargin, instantaneous administration of Jujuboside B induced a rapid and intense Ca²⁺ influx. (C) Representative confocal image showed that after depleting intracellular Ca²⁺ store by thapsigargin, SKF96365 inhibited Jujuboside B-induced Ca²⁺ influx. (D) Fluorescence intensity curves showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca²⁺ elevation, n = 4. (E) Analysis of intracellular Ca²⁺ concentration showed that SKF96365 significantly inhibited Jujuboside B-induced intracellular Ca²⁺ elevation, ** P < 0.01 compared with Control, ## P < 0.01 compared with Jujuboside B, n = 4.