. 2016 Feb 23;7:167. doi: 10.3389/fmicb.2016.00167

Table 4.

T. paralvinellae and P. furiosus specific enzyme activities.

Enzyme^†	Media type						p-value
	0.5% maltose	0.5% tryptone	0.5% maltose 0.05% tryptone	0.5% maltose + 10 mM acetate	0.5% maltose (pH 5)	1% waste milk
T. paralvinellae
Mbh	3.34 ± 0.91	4.62 ± 2.20	1.53 ± 0.31	1.36 ± 0.29	0.18 ± 0.18	ND	NS
Sulf	0.21 ± 0.03^a	0.54 ± 0.20^a	1.53 ± 0.15	0.06 ± 0.01^a	0.05 ± 0.01^a	0.35 ± 0.14^a	^***
ATPase	0.23 ± 0.04^a^,^b	3.30 ± 0.44^d	0.39 ± 0.29^a^,^c	4.41 ± 0.03^d	1.33 ± 0.20^b^,^c	ND	^***
FNOR	0.21 ± 0.07^a	3.42 ± 0.16^c^,^d	3.33 ± 0.60^b^,^c	9.10 ± 1.02	1.84 ± 0.22^a^,^b^,^d	1.71 ± 0.22^a^,^b^,^d	^***
GDH	12.09 ± 0.75^a	9.03 ± 0.56^a^,^c	3.88 ± 1.26^b	3.89 ± 0.91^b	8.31 ± 0.30^c	0.53 ± 0.14^b	^***
AlaAT	1.21 ± 0.47^a	5.80 ± 2.46^a^,^b	5.08 ± 1.27^a^,^b	3.01 ± 0.11^a^,^b	8.00 ± 0.37^b	2.42 ± 0.45^a^,^b	^*
POR	2.59 ± 0.13^a	0.46 ± 0.10^b	1.29 ± 0.09	0.41 ± 0.05^b	2.16 ± 0.40^a	0.18 ± 0.05^b	^***
VOR	0.34 ± 0.07^a	0.11 ± 0.03^b	0.44 ± 0.05^a	0.02 ± 0.01^b	0.11 ± 0.03^b	0.14 ± 0.03^b	^***
ACS	0.34 ± 0.07^a	0.52 ± 0.02^b	0.60 ± 0.03^b	0.21 ± 0.02^a^,^c	0.60 ± 0.02^b	0.13 ± 0.06^c	^***
AOR	0.35 ± 0.05^a	0.48 ± 0.09^a	0.29 ± 0.05^a	0.60 ± 0.10^a	1.55 ± 0.43	0.10 ± 0.03^a	^***
FOR	0.28 ± 0.10^a^,^b	0.62 ± 0.14^b	0.12 ± 0.03^a	0.34 ± 0.13^a^,^b	0.46 ± 0.06^a^,^b	0.16 ± 0.02^a	^**
ADH	0.03 ± 0.01^a^,^b	0.02 ± 0.00^a^,^b	0.07 ± 0.03^a	0^b	0.02 ± 0.01^a^,^b	0^b	^**
FDH	0.06 ± 0.02^a	0.03 ± 0.00^a^,^b	0.02 ± 0.00^a^,^b	0.04 ± 0.01^a^,^b	0.02 ± 0.00^a^,^b	0.01 ± 0.01^b	^*
P. furiosus
Mbh	4.99 ± 0.31^a	9.55 ± 0.50^a^,^b	12.74 ± 0.24^b	11.42 ± 0.19^b	5.39 ± 0.40^a^,^b	ND	^**
Sulf	1.27 ± 0.11^a^,^a^,^c	0.73 ± 0.19^a^,^a^,^d	3.44 ± 0.49	1.48 ± 0.29^a^,^a^,^e	0.15 ± 0.05^d^,^f	0.52 ± 0.02^c^,^c^,^f	^***
ATPase	0.05 ± 0.00^a	0.01 ± 0.01^a	0.70 ± 0.22	0^a	0^a	ND	^*
FNOR	6.10 ± 0.60^a	3.90 ± 0.32^a^,^b	4.59 ± 0.48^a	3.83 ± 0.47^a^,^b	0.31 ± 0.12^b	3.16 ± 1.59^a^,^b	^***
GDH	1.85 ± 0.36^a	9.90 ± 1.08^c	4.69 ± 0.42^b	0.69 ± 0.08^a	0.91 ± 0.09^a	0.68 ± 0.16^a	^***
AlaAT	0.54 ± 0.25	3.05 ± 0.61	2.59 ± 1.76	0.72 ± 0.14	0.36 ± 0.05	0.54 ± 0.29	NS
POR	2.27 ± 0.43	0.93 ± 0.10^a	0.72 ± 0.03^a	0.85 ± 0.09^a	0.30 ± 0.02^a	0.01 ± 0.01^a	^***
VOR	1.88 ± 0.23^a	2.01 ± 0.45^a	0.56 ± 0.12^b^,^c	0.56 ± 0.06^b^,^d	1.35 ± 0.05^a^,^a^,^d	0.11 ± 0.05^c^,^d	^***
ACS	0.87 ± 0.09^a^,^a^,^c	1.30 ± 0.12^b	1.15 ± 0.24^a	0.68 ± 0.09^a^,^c	0.57 ± 0.04^a^,^c	0.44 ± 0.19^c	^***
AOR	0.36 ± 0.04^a	0.12 ± 0.03^b^,^c^,^d	0.24 ± 0.01^a^,^b^,^d	0.26 ± 0.07^a^,^c	0.08 ± 0.02^d	0.06 ± 0.02^d	^***
FOR	0.47 ± 0.08	0.27 ± 0.06	0.36 ± 0.01	0.45 ± 0.11	0.31 ± 0.09	0.47 ± 0.23	NS
ADH	0	0	0.01 ± 0.00	0	0	0.01 ± 0.01	NS
FDH	0.02 ± 0.00	0.06 ± 0.02	0.02 ± 0.01	0.01 ± 0.01	0.03 ± 0.01	0	NS

^†

The specific activities (U [mg protein]⁻¹) are the averages of no less than three technical replicates and two biological replicates (±standard error). ND, a specific activity was not determined. p-values calculated from one-way ANOVA test comparing enzymatic activity across treatments.

p < 0.05,

^**

p < 0.01, and

^***

p < 0.001.

^{a, b, c, d, e, f}

Tukey post-hoc test indicates groups whose members are not significantly different. NS, no significance.