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. 2016 Feb 22;11(2):e0149497. doi: 10.1371/journal.pone.0149497

Fig 6. VPA and TSA delay the formation of pharyngeal teeth.

Fig 6

The development of pharyngeal teeth was analyzed at 72 hpf, 96 hpf and 120 hpf. GFP fluorescence in embryos of the Dlx2b-EGFP transgenic line: controls (A-C), embryos treated with 1 μM VPA (D-F) or 0.3 μM TSA (G-I). White arrows indicate pharyngeal tooth germs. Alcian blue staining at 144 hpf in control embryos (J) or embryos treated with 0.3 μM of TSA (K) or 5 μM of VPA (L). Dotted lines highlight the contours of three teeth: 3V1, 4V1 and 5V1.