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. 2016 Feb 23;7:50. doi: 10.3389/fphys.2016.00050

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Copyright © 2016 Akita, Kano, Saito-Tamura, Mashimo, Sato-Shionome, Tsurumachi, Yamanaka, Kaneko, Toriumi, Arai, Tsukimura, Matsumoto, Ishigami, Isokawa and Honda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of fluorescent-labeled ASCs and DFAT cells analyzed via fluorescence microscopy 5 weeks after transplantation. (A,C,E,G) Highly magnified fluorescence images of the fenestration defect in the distal root of the first molar. (H&E staining; scale bar: 50 μm). (B,D,F,H) The dotted line indicates newly formed periodontal ligament tissue between cementum and bone. Fluorescent-labeled ASCs and DFAT cells were observed in periodontal ligament beside the bone (arrows) and cementum (arrowheads). (Blue color: DAPI; Scale bar: 50 μm.). NB, newly formed bone; Ce, newly formed cementum; De, root dentin.