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. 2016 Feb 23;7:50. doi: 10.3389/fphys.2016.00050

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Copyright © 2016 Akita, Kano, Saito-Tamura, Mashimo, Sato-Shionome, Tsurumachi, Yamanaka, Kaneko, Toriumi, Arai, Tsukimura, Matsumoto, Ishigami, Isokawa and Honda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multilineage potential of DFAT cells and ASCs. (A) ALP activity and Alizarin red staining, examined to detect osteogenic potential of DFAT cells, and ASCs. (B) Calcium concentration determined by quantitative colorimetric assay. Each bar represents the mean ± SD (n = 12); ^*P < 0.05. (C) Cells stained for Alcian blue to determine chondrogenic differentiation. Scale bar = 100 μm. (D) Adipogenic differentiation evaluated by staining with Oil red O. (E) Oil red O-positive lipid droplets were measured in DFAT cells and ASCs under growth and adipogenic induction media. There was no significant difference between DFAT cells and ASCs on the cell number stained by Oil red O.