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. 2016 Feb 23;7:178. doi: 10.3389/fmicb.2016.00178

FIGURE 4.

FIGURE 4

Mean fluorescence intensity of endogenous NO generated during germination at different time points after different treatments. Bright fluorescence was observed after treatment with distilled water (H2O) and DAF-FM DA (A); faint fluorescence was observed in distilled water without DAF-FM DA staining (CK) (B). U, urediniospore; B, base of germ tube; A, apex of germ tube. c+D, c-PTIO +DPI; L+N, L-Arg +NADPH; L+D, L-Arg +DPI; c+N, c-PTIO +NADPH.