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. 2016 Feb 23;7:178. doi: 10.3389/fmicb.2016.00178

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Copyright © 2016 Yin, Gao, Wang, Huang, Kang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mean fluorescence intensity of endogenous ROS generated during germination at different time points and treatments. Bright fluorescence was observed after treatment with distilled water (H₂O) and H₂DCF DA (A); faint fluorescence was observed in distilled water without H₂DCF DA staining (CK) (B). U, urediniospore; B, base of germ tube; A, apex of germ tube. c+D, c-PTIO +DPI; L+N, L-Arg +NADPH; L+D, L-Arg +DPI; c+N, c-PTIO +NADPH.