Table 1. Detail of criteria used for digital analysis of Surface Area and Volume of 3D models.
| Category | Criteria | Surface Area | Volume |
|---|---|---|---|
| Underwater | Unsampled | Complete 3D model of unsampled colony after segmentation from community | Complete 3D model of unsampled colony after segmentation from community |
| TSA/TV Sampled | Complete 3D model of sampled colony after segmentation from community | Complete 3D model of sampled colony after segmentation from community | |
| ESA Sampled | 3D model of sampled colony after segmentation of non-living contact areas | Not applicable | |
| Dry | TSA/TV Tissue | Complete 3D model of fresh sample after segmentation from imaging stage | Complete 3D model of fresh sample after segmentation from imaging stage |
| ESA Tissue | 3D model of fresh sample after segmentation of non-living contact areas | Not applicable | |
| TSA/TV Skeleton | Complete 3D model of skeleton sample after segmentation from imaging stage | Complete 3D model of skeleton sample after segmentation from imaging stage | |
| ESA Skeleton | 3D model of skeleton sample after segmentation of non-living contact areas | Not applicable | |
| TV Water | Not applicable | Immersion of skeleton sample in water | |
| Analysis | ThSA/ThV | Unsampled SA minus ESA Sampled | Unsampled V minus TV Sampled |
| Biosurface/Biomass | TSA Tissue minus TSA Skeleton | TV Tissue minus TV Skeleton | |
| % Scale | % of ESA Tissue compared to Unsampled | % of TV Tissue compared to Unsampled | |
| Errors | Theoretical Error | Calculated error of ThSA compared to ESA Tissue | Calculated error of ThV compared to TV Tissue |
| Error TVW | Not applicable | % of TV Water compared to TV Skeleton |
TSA (Total Surface Area), TV (Total Volume), ESA (Effective Surface Area), ThSA (Theoretic Surface Area), ThV (Theoretic Volume), Error TVW (Total Volume Water).