FIGURE 6.
Real-time RT-PCR analysis of PR1, PR2, PR5, PDF1.1, and PDF1.2 gene expression in Arabidopsis leaves (A), roots (B), flowers (C), and pods (D) upon a treatment with U. virens. Arabidopsis leaves and roots were inoculated by adding a suspension of germinated microconidia in order to reach the concentration of 5 × 105 conidia mL-1. Control leaves, flowers, roots, and pods were treated with sterile distilled water. Expression levels were normalized with respect to the housekeeping gene Actin. Data bars represent the mean ± SD of three repeats of the transcripts assessed at 0, 24, 48, 72, and 96 h post-inoculation for leaves and roots while flowers and pod gene transcripts were assessed 5 weeks post inoculation.