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(a) EMSA results for rs386770867 using BEAS-2B, Calu-3 and Daudi nuclear extracts A total of 5 μg of nuclear extract was used per reaction. (b) Competition EMSA using Calu-3 nuclear extract. Excess of cold probe used was 10x, 50x and 100x with 5 μg of nuclear extract used per reaction. (c) Competition EMSA using 5 μg of Daudi nuclear extract and 10x, 50x and 100x excesses of cold probe. (d) Supershift assay using 5 μg of Daudi cell nuclear extract per reaction.
