Figure 2. Expression levels of genes after heat shock treatment.

Cultures of A. sinensis cells in suspension were subjected to heating to 50 °C for 30 min. After this heat shock treatment, the culture flasks were returned to the gyratory shaker at 25 °C for continued shaking and were sampled at appointed times (0 h, 0.5 h, 2 h, 4 h, 6 h, 12 h, 24 h, and 3d). Healthy cells in suspension culture that were not subjected to heat shock treatment were used as controls. Expression levels of AsLOX, AsAOS, AsAOC and AsMYC2 were assayed using real-time PCR analysis and AsGADPH as the internal control. All genes were up-regulated in response to heat shock treatment. Each value is the mean ± SE of 3 independent biological replicates.