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. 2016 Feb 23;6:21843. doi: 10.1038/srep21843

Figure 3. Time course of endogenous jasmonates induced by heat shock treatment.

Figure 3

A. sinensis cells cultured in suspension containing or not containing 1.0 μM nordihydroguaiaretic acid (NDGA), an inhibitor of JA biosynthesis, were subjected to heating at 50 °C for 30 min. After the heat shock treatment, the culture flasks were returned to the gyratory shaker at 25 °C for continued shaking and were sampled at appointed times (0 h, 0.5 h, 2 h, 4 h, 6 h, 12 h, 24 h, and 3d). Healthy cells cultured in suspension that did not receive heat shock treatment were used as controls. Endogenous jasmonates were extracted and analyzed by GC-MS. Heat shock treatment rapidly induced JA production in A. sinensis cell suspensions, and NDGA, a specific inhibitor of JA, could block this induction, as demonstrated by the lack of JA accumulation. CK indicates the healthy control; Heat Shock indicates NDGA-free treatment in a 50 °C water bath; Heat Shock + NDGA indicates NDGA treatment in a 50 °C water bath.