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. 2016 Feb 23;6:21815. doi: 10.1038/srep21815

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Copyright © 2016, Macmillan Publishers Limited

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An annexin-V fluorescein APC/7-AAD double stain assay and flow cytometry analysis were performed to confirm cell apoptosis and to explore the differences in the apoptosis induction resulting from these four mutations versus the wild type. The lower left quadrant represents vital cells. The number of early apoptosis cells and late apoptosis cells was indicated in lower right quadrant and upper right quadrant of the histograms, respectively.