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. 2016 Feb 23;6:21865. doi: 10.1038/srep21865

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Copyright © 2016, Macmillan Publishers Limited

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(A) Sirt1 AS overexpression in myoblasts. GAPDH was employed as an internal reference. (B) miR-34a overexpression. (C) The levels of Sirt1 mRNA in myoblasts transfected with p-Sirt1 AS (pCDNA3.1-Sirt1 AS), p-miR-34a (pCMV-miR-34a) and p-Sirt1 AS plus p-miR-34a. (D,E) The levels of Sirt1 protein in myoblasts transfected with p-Sirt1 AS, p-miR-34a and p-Sirt1 AS plus p-miR-34a. The levels of Sirt1 protein were detected by western blotting. GAPDH protein was applied as internal reference. The data were presented as means ± SEM of 4 independent experiments. *P < 0.05 and **P < 0.01.