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. 2016 Feb 23;6:21865. doi: 10.1038/srep21865

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(A–C) The mRNA levels of CyclinB, CyclinD and CyclinE were upregulated in C2C12 cells of Sirt1 AS overexpression, but downregulated in miR-34a overexpression at 24 hours after treatment with growth medium. (D,E) The levels of CyclinB, CyclinD and CyclinE proteins in C2C12 cells of Sirt1 AS, miR-34a and Sirt1 AS plus miR-34a overexoression at 24 hours after induced with growth medium. Results were indicated as the mean ± SEM of 4 independent experiments. *P < 0.05.