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. 2016 Feb 23;6:21865. doi: 10.1038/srep21865

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(A–C) Sirt1 AS overexpression downregulated the levels of myogenic gene (MyoD, MyoG and MHC) mRNA and protein. (D) Immunocytochemical staining. (E,F) The quantification analysis of MyoG and MHC positive nuclei. The data were presented as means ± SEM of 4 independent experiments. *P < 0.05.