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(A) Schematic of Sirt1 3′ UTR inserted into psiCHECK™-2 vector. (B) Luciferase activity analysis for Sirt1 AS, Sirt1 and miR-34a. (C) Sirt1 AS lncRNA makes Sirt1 mRNA much more stable. The data were presented as means ± SEM of 4 independent experiments. *P < 0.05 and **P < 0.01.
