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. 2016 Feb 23;6:21865. doi: 10.1038/srep21865

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(A) Body weight. (B) Body composition analysis. (C) HE staining. (D) Levels of Sirt1 mRNA. (E) Levels of Sirt1 AS lncRNA. (F) Levels of miR-34a. (G/H) Levels of Sirt1, CyclinB, CyclinD, CyclinE, MyoD, MyoG and MHC proteins. The results were presented as means ± SEM of at least 4 independent experiments. *P < 0.05, **P < 0.01. (I) Model for Sirt1 AS lncRNA fully bound to Sirt1 mRNA to regulate muscle formation by competing with miR34a. OE: Sirt1 AS overexpression, Quad: quadriceps, Sol: soleus, Gast: gastrocnemius, Ing: inguinal fat.