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. 2016 Feb 23;6:22034. doi: 10.1038/srep22034

Figure 3. Resveratrol attenuates AR–stimulated proliferation by activating SIRT1.

Figure 3

(A) Cell viability was measured by MTT assay following treatment of MCF-7, T47D, and SK-BR-3 cells with resveratrol at indicated concentrations for 48 h. (B) Cell viability was measured by MTT assay following treatment with resveratrol at the indicated concentrations, for 24, 36, 48, or 60 h. (C) Cell apoptosis was detected by flow cytometry analysis after 40 μM resveratrol treatment. (D,E) SIRT1, mTOR, AR, and Skp2/B protein levels were determined by western blotting and analyzed by grayscale software following treatment with 40 μM resveratrol for 48 h, and normalized to GAPDH expression. (F,G) SIRT1 and AR protein levels were determined by western blotting and analyzed by grayscale software following treatment with 40 μM resveratrol and/or transfection with siSIRT1 for 48 h, and normalized to GAPDH expression. Data in are the mean of three independent experiments. Res: resveratrol. *P < 0.05, as compared with untreated cells.