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(a) 48-hour wild-type biofilms treated with 10 μg/ml amphotericin B (AmB) for 24 hours (gray) or untreated (black). Viability was measured as colony-forming units (CFUs). Surviving populations were reinoculated to form new biofilms that were re-exposed to AmB for three cycles. (b) Survival of 48-hour wild type biofilms treated for 24 hours with 10, 50, or 100 μg/ml AmB. n = 3, error bars show standard deviation.
