Table 1. Mutants enriched in the barcode-sequencing screen after amphotericin B treatment.
| Biological Function | Growth Modes for Mutant Identification |
||
|---|---|---|---|
| Biofilm | Planktonic | Biofilm+Planktonic | |
| Lipid metabolism | tgl1 cki1 | ncp1 inp51 ura7 ecm22 | sac1 erg5 cyb5 tlg2 |
| Translation | rpp1b ssz1 | rps22b rpl40b rpl42a rps17b rpl6b rpl24a | rpl8b rps21a |
| Protein metabolism | hrt3 mnn4 | rpn10 ste14 glr1 ubc4 ptc7 | eug1 doa4 jem1 |
| RNA metabolism | brr1 pet127 isu1 ngl2 | set2 ssn2 sas2 gbp2 syf2 ski2 hbs1 ynr024w sfl1 tea1 vts1 nut1 edc3 pih1 pnc1 nsr1 trm11 yll029w nam7 | ssn3 |
| Cell cycle | ptc2 bud9 ndt80 mrc1 gic1 hsl7 | rme1 msc7 far8 pcl9 ama1 | elm1 gin4 |
| Intracellular transport | shr5 ypt7 pep3 age2 | srp101 aps3 pex21 tom5 jjj1 fmp37 vtc1 fmp43 btn2 vps13 arx1 yer128w xdj1 | osh2 vta1 apm3 |
| Small molecule metabolism | ypl033c | trp4 bna2 gcv1 | sah1 ykl151c |
| TORC1 signaling | gtr2 | gtr1 meh1 slm4 slm1 | |
| Other functions | gal83 mdh2 pck1 sak1 ynr064c srl2 pbp2 YBL055C | cis3 spo71 dit2 qcr8 rip1 yjl055w inp1 ylr073c pgm2 ymr099c ynl191w mms2 gyp1 arn1 sam3 | ras2 sma2 ybr238c rho3 izh3 vht1 zrt1 |
| Unknown | ylr283w om45 dlt1 ylr064w ylr278c ybr255w ypl068c | ecm27 pib2 ynl208w yal049c ylr125w rmd8 met12 mpa43 ytp1 ymr181c ylr407w ygr259c yor292c nrp1 ybr016w yor139c ybl081w tma16 | asg1 ygr182c ygr190c yhr159w |
Mutants significantly more abundant in mixed populations of knockout mutants after treatment with amphotericin B in biofilm and planktonic populations. Mutants are classified according to the gene ontology of the corresponding wild type alleles by biological processes. See Supplementary Table S2 for more details.