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. 2016 Feb 23;6:21671. doi: 10.1038/srep21671

Figure 4. The Kat1 BED-domain is important for DNA binding in vitro.

Figure 4

Electrophoretic mobility shift assay (EMSA) using a 577-bp DNA probe including both TIR-R and TIR-L and increasing amounts of (a) GST-empty vector, GST-Kat1, GST-Kat1-C and GST-Kat1-N. Free probe, protein-DNA complexes and slots are indicated. (b,c) as in (a), but using increasing amounts of GST-Kat1C130A/C133A and GST-Kat1C402A/H405A. In each reaction ~1.4 nM of substrate was incubated with ~5, 10, 20, and 40 nM of Kat1 for 1 h at 4 °C. Reaction products were analyzed by 4% native PAGE.