Table 1. Comparisons of this study to several reported biotin detection methods.

Detection methods	LOD	references
Aptamer as a competitor	ST-21 16.48 pg/ml	this study
	ST-21M 5.83 pg/ml
ELISA	977 pg/ml	8
Electrochemical immunosensor	2.02 ng/ml	15
Electrochemical magneto	20 ng/ml	14
LC	1–9 ng/g	7
HPLC/MS	2.5 ng/ml	27
Flow injection liposome immunoanalytical system	250 pg/ml	9
Immunoaffinity chromatographic	10 pg/ml	10
Isotope dilution	1 ng/ ml	13
Kinetic spectrophotometry	180 ng/ml	28
Surface plasmon resonance	0.5 ng/ml	11