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. 2016 Feb 23;6:22066. doi: 10.1038/srep22066

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Copyright © 2016, Macmillan Publishers Limited

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(A–D) Effects of Pygo2 knockdown by shRNAs on the expressed of H3K4me2 and -3, present on the promoters of CD44 and CyclinD1, in human brain glioma U-87MG (A) and U251 (B) cell lines. The enrichment of H3K4me2/3 was measured by CHIP. Histone H3 was used as a negative control. (E,F) Effects of Pygo2 knockdown by shRNAs on the total protein expression of H3K4me2 and -3 in human brain glioma U-87MG (E) and U251 (F) cell lines. The protein levels were measured by western blot assay, with histone H3 used as a negative control. Arrows (→) indicate cropping lines and gels were obtained under the same experimental conditions. **P < 0.01 and ***P < 0.001.