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. 2016 Feb 23;6:22192. doi: 10.1038/srep22192

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Copyright © 2016, Macmillan Publishers Limited

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(A) Quantitative RT-PCR analysis of PI3K, Akt, ERK, miR-126 and Let-7. Data are the mean ± SD of three separate experiments. Statistical significance was determined by the Mann–Whitney test (p < 0.05). (B) Immunoblot analysis of PI3K/Akt and ERK signalling molecules. Decreased expression of PI3K, p-Akt^Ser473 and p-ERK1/2^{Thr202/Tyr204} observed in nimbolide treated animals compared to DMBA painted hamsters. Gapdh was used as loading control for cytosol. Phosphorylated proteins are normalized by their unphosphorylated forms. (C) Background subtracted protein bands quantified and normalized to Gapdh. Phosphorylated proteins are normalized by their unphosphorylated forms. Each bar represents the protein expression ± SD of three determinations. ^♣significantly different from control (p < 0.05). *significantly different from DMBA-treated group (p < 0.05).