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. 2016 Feb 23;6:21934. doi: 10.1038/srep21934

Figure 9. The differential expression of cerato ulmin gene after treatments with plant defense elicitors using M75-LUC and M75-GUS reporter systems.

Figure 9

(a) M75-LUC (L8) transgenic fungal strain was used to observe the induction kinetics of cerato ulmin gene in liquid media treated with 0.01% ethanol (Control), SA (1, 2, 4 mM) or MeJA (25, 50, 100 μM). The relative luminescence units (RLU) were recorded for each sample every 24 h and for 144 h after treatment. Values represent the mean ( ± SE) of three biological replicates. (b) The fresh weight (gm) of fungal pellet was determined after treatment of fungal liquid media with 0.01% ethanol (Control), SA (1, 2, 4 mM) or MeJA (25, 50, 100 μM) for 144 h. Values marked with the same letter are not significantly different (P < 0.05). (c) M75-GUS (L4) transgenic fungal strain was used to observe the differential expression of cerato ulmin in solid media treated with 5 ml of 0.01% ethanol, SA (2 mM) or MeAJ (50 μM) for 24 h and then stained with GUS staining solution for 24 h at 37 °C. Each plate contains four mycelial plugs of (M75-GUS) strain and one mycelial plug of (M75-LUC) strain (negative control). (d) The expression of On-Cerato-ulmin gene was quantified in fungal tissues grown in liquid medium treated with 0.01% ethanol (Control), SA (2 mM) or MeJA (50 μM) for 122 h. The expression of On-Cerato-ulmin was examined in three strains of O. ulmi (H200-O, H956, Q412T-O) and O. novo-ulmi (VA-3 O, FG245-O, MH75-4 O). Values represent the mean (±SE) of four biological replicates. The expression of On-Cerato-ulmin was normalized to that of On-Actin in the same sample and was calculated relative to the expression of On-Cerato ulmin in H200-O control sample. Values marked with an asterisk (*) are significantly lower (≤4-times) compared to the control treatment (P < 0.05).