Figure 1. Phylogenetic inference of the tick egg associated bacteria using 16S rRNA gene sequences. Neighbor-Joining analysis with Kimura 2-parameter based on the nucleotide sequences was performed. Arrows indicate sequences obtained in the present work, and the GenBank accession codes for other sequences are presented in parenthesis. Sequences were aligned using the ClustalW program (Promega, Madison, WI), and phylogenetic inferences obtained using the MEGA 5.2.2 software. Internal node supports were calculated using bootstrap analyses with 1,000 replicas. Bootstrap values below 70% are not present.
