Figure 4. Knockdown of NDRG1 relieves the VPA-mediated suppression of PC3 invasiveness. (A) PC3 cells were transfected with NDRG1 shRNA or treated with 1 mM VPA for 24 h as indicated. Protein levels of E-cadherin and NDRG1 were monitored by western blotting. (B) The same amount of Mock vector control or NDRG1 shRNA transfected PC3 cells was seeded on a matrigel coated chamber and treated with 1 mM VPA. After 22 h, the cells that had invaded the lower surface of the membrane were counted. Values are expressed as means ± SD of three independent experiments, and the p value shown is from a Student's t-test analysis. (C) Microscopic image of matrigel invaded cells stained with Giemsa from the experiment shown in (B).
