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. 2016 Feb 22;12(2):e1005461. doi: 10.1371/journal.ppat.1005461

Fig 3. Selective SP-D binding to L4 Nippostrongylus brasiliensis enhances host protective immunity.

Fig 3

Confocal microscopic images of L3, L4 and Adult stage of N. brasiliensis incubated with 20 μg/ml of rfhSP-D, biotin-conjugated anti-rfhSP-D, followed by streptavidin-cy3 (a). 250 L4 stage larvae, coated or uncoated with rfhSP-D were intra-nasally administered into naïve mice. Intestinal worm burden (b), detection by ELISA of IL-33, TSLP and IL-25 in the lung (c), percentage of lung ILC2 and mean fluorescence intensity (MFI) of markers of alternative macrophage activation were quantified at day 5 PI. (d). Data are representative of 4 individual experiments. N = 5–8 mice per group. *P<0.05, **P<0.01.