Fig 3. Effect of daidzein on PPAR-α and PPAR-γ activity in HEK293T cells.

HEK293T cells were transfected with PPRE-containing reporter plasmid and (A) PPAR-α or (B) PPAR-γ expression plasmids, and then treated with the indicated concentrations (6.25–25 μM) of daidzein for 24 h. Luciferase activity was measured and expressed as a fold induction, that was corrected for transfection efficiency using renilla luciferase activity. Values are expressed as the fold change compared with the vehicle control that was arbitrarily set to 1. Δ, p<0.1; *, p<0.05; **, p<0.01 versus non-treated control.