TABLE 1.
Fold of IS |
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RNA-Seq |
Real-time PCR |
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Iron status × choline2 |
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Gene | IS-C | FID | FID-C | IS-C | FID | FID-C | P value | F value |
ApoE | 0.91 | 0.86* | 0.93 | 0.76*** | 0.73*** | 0.99 | <0.0001 | 42.2 |
Cartpt | 0.58** | 0.50*** | 0.45*** | 0.94 | 0.58 | 1.15 | 0.08 | 3.58 |
Drd1a | 1.08 | 1.22 | 1.00 | 1.18 | 1.39** | 1.07 | 0.0005 | 16.6 |
Gas5 | 0.50** | 0.64 | 0.80 | 0.35*** | 0.44** | 0.66*** | <0.0001 | 114 |
Gda | 1.19** | 1.24*** | 1.06 | 1.33*** | 1.58*** | 1.14 | <0.0001 | 139 |
Hcrt | 0.74 | 0.57* | 1.00 | 0.75 | 0.44** | 1.07 | 0.06 | 4.17 |
Igf1 | 1.03 | 1.33* | 1.06 | 1.04 | 1.20** | 0.96 | 0.0066 | 8.98 |
Mbp | 0.94 | 0.86* | 1.04 | 0.64** | 0.61*** | 0.79 | 0.0002 | 22.2 |
Pld5 | 1.27 | 2.57*** | 1.89*** | 1.39** | 2.35*** | 1.11 | <0.0001 | 40.6 |
Pmf1 | 0.95 | 0.47* | 0.96 | 0.61*** | 0.62*** | 0.74** | <0.0001 | 90.5 |
Values are means (n = 4 for RNA-Seq and n = 5 for real-time PCR). *, **, ***Different from IS: *P < 0.05, ** P < 0.01, ***P < 0.001. FID, formerly iron deficient; FID-C, FID-choline; IS, iron sufficient; IS-C, IS choline; PND, postnatal day. A list of definitions of gene and protein names used in this table is included in Supplemental Material.
Two-factor ANOVA.