TABLE 1.

Real-time (TaqMan) PCR validation of RNA-Seq data of PND65 rat hippocampal transcriptome following gestational iron deficiency with or without choline supplementation¹

	Fold of IS
	RNA-Seq			Real-time PCR
							Iron status × choline2
Gene	IS-C	FID	FID-C	IS-C	FID	FID-C	P value	F value
ApoE	0.91	0.86*	0.93	0.76***	0.73***	0.99	<0.0001	42.2
Cartpt	0.58**	0.50***	0.45***	0.94	0.58	1.15	0.08	3.58
Drd1a	1.08	1.22	1.00	1.18	1.39**	1.07	0.0005	16.6
Gas5	0.50**	0.64	0.80	0.35***	0.44**	0.66***	<0.0001	114
Gda	1.19**	1.24***	1.06	1.33***	1.58***	1.14	<0.0001	139
Hcrt	0.74	0.57*	1.00	0.75	0.44**	1.07	0.06	4.17
Igf1	1.03	1.33*	1.06	1.04	1.20**	0.96	0.0066	8.98
Mbp	0.94	0.86*	1.04	0.64**	0.61***	0.79	0.0002	22.2
Pld5	1.27	2.57***	1.89***	1.39**	2.35***	1.11	<0.0001	40.6
Pmf1	0.95	0.47*	0.96	0.61***	0.62***	0.74**	<0.0001	90.5

¹Values are means (n = 4 for RNA-Seq and n = 5 for real-time PCR). *, **, ***Different from IS: *P < 0.05, ** P < 0.01, ***P < 0.001. FID, formerly iron deficient; FID-C, FID-choline; IS, iron sufficient; IS-C, IS choline; PND, postnatal day. A list of definitions of gene and protein names used in this table is included in Supplemental Material.

²Two-factor ANOVA.