Table 4. Reactions and rate constants for dynamic recruitment in the model.
X indicates cAMP bound or not to the catalytic site, in addition, these reactions occur for both the phosphorylated and unphosphorylated PDE4D forms. Y indicates PDE4D bound or not to AP. Z indicates PDE4D bound or not to APcyt. APcyt is the unidentified anchoring protein for PDE4D in the cytosol, and APsm is the submembrane anchoring protein. APsm_PDE4D is the plasma membrane PDE4D in Fig 5.
| Reaction | kf (nM-1 ms-1) | kb (ms-1) | kcat (ms-1) | source |
|---|---|---|---|---|
| APcyt_PDE4D_X + 2 cAMP ↔ APcytcAMP2_PDE4D_X | 8.70E-08 | 0.00002 | adj | |
| APcytcAMP2_PDE4D_X + 2 cAMP ↔ APcytcAMP4_PDE4D_X | 1.15E-07 | 0.0002 | adj | |
| APcytcAMP4_PDE4D_X ↔ APcytcAMP4 + PDE4D_X | 1.60E-05 | 1.70E-06 | adj | |
| PDE4D_X + APsm ↔ APsm_PDE4D _X | 1.70E-06 | 1.60E-05 | adj | |
| PKAc + Y_PDE4D_X ↔ Y_PKAcPDE4D_X → Y_pPDE4D_X + PKAc | 4.28E-07 | 0.00056 | 0.000125 | same as other PDE4 |
| Z_PDE4D + cAMP ↔ Z_PDE4DcAMP → Z_PDE4D + AMP | 2.166E-05 | 0.06895 | 0.017233 | same as other PDE4 |
| Z_pPDE4D + cAMP ↔ Z_pPDE4DcAMP → Z_pPDE4D + AMP | 4.332E-05 | 0.1379 | 0.034467 | same as other pPDE4 |
| APsm_PDE4D + cAMP ↔ APsm_PDE4DcAMP →APsm_PDE4D + AMP | 2.166E-04 | 0.6895 | 0.17233 | 10x case in Fig 5 |
adj: adjusted to effect