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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Exp Eye Res. 2015 Aug 25;140:85–93. doi: 10.1016/j.exer.2015.08.013

Fig. 3.

Fig. 3

The influence of TRPV4 antagonist HC067047 (panel A) and SFK inhibitor PP2 (Panel B) on SFK activation in the epithelium of lenses subjected to freeze-thaw (FT) damage. Lenses were preincubated with or without 10 μM HC067047 (20 min), or 10 μM PP2 (40 min), subjected to the FT maneuver, then incubated for a further 2 min in the continued presence or absence of HC067047 or PP2, respectively. Control lenses were not damaged. On the left of each panel the typical western blot shows pY416-SFK (upper) and β-actin or GAPDH (lower) which was used as loading control. The bar graphs (right on both panels) show densitometric analysis of pY416-SFK normalized to β-actin/GAPDH. The results are the mean ± SEM of data from 3 independent experiments. ** (p<0.01) indicates a significant difference compared to control and ### (p<0.001) indicates a significant difference compared to the FT-damage group.