The influence of HC067047 (upper panel) and PP2 (lower panel) on Na,K-ATPase activity in the epithelium of lenses that received 5 μl of hyperosmotic NaCl solution (2400 mOsm) injected ~1 mm beneath the capsule of the posterior pole. Lenses were preincubated with or without 10 μM HC067047 (20 min), or 10 μM PP2 (40 min), subjected to the 5 μl injection, then incubated for a further 5 min in the continued presence or absence of HC067047 or PP2. Control lenses did not receive an injection. The results are the mean ±} SEM of data from 3 independent experiments. ** (p<0.01) and *** (p<0.001) indicates significant differences compared to the Control group, while ### (p<0.01) indicates a significant difference compared to the NaCl injection group. Levene’s test show homoscedasticity of data for all groups in panel A (p=0.459) and panel B (p=0.218).