Fig. 2.

Drug-induced DNA cleavage mediated by WT and mutant M. tuberculosis gyrase. The ability of WT (black), GyrA^A90S (A90S, blue), GyrA^A90V (A90V, red), GyrA^D94H (D94H, green), and GyrA^D94G (D94G, yellow) to mediate DNA cleavage in the presence of the clinically used fluoroquinolones ciprofloxacin and moxifloxacin (Top Left and Top Right, respectively), the experimental quinazolinediones 8-methyl-3′-(AM)P-dione and 8-H-3′-(AM)P-dione (Middle Left and Bottom Left, respectively), and the experimental fluoroquinolones 8-methyl-3′-(AM)P-FQ and 8-H-3′-(AM)P-FQ (Middle Right and Bottom Right, respectively) is shown. Compound structures are shown in or above their respective panels. Error bars represent the SD of at least three independent experiments.