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. 2016 Feb 1;113(7):1937–1942. doi: 10.1073/pnas.1523645113

Fig. 1.

Fig. 1.

Melanopsin regulates mTOR activity in RGCs. (A) Whole-mount retinas from Opn4-GFP mice with intact optic nerves or at 1 d post injury (1DPI), with Tuj1 (red), pS6 (blue), and GFP (green) staining. (B and C) Quantification of the percentages of Tuj1+ RGCs (B) or GFP+ ipRGCs (C) expressing pS6. *P < 0.05, Student’s t test, three mice in each group. (D) Whole-mount retinas from Opn4−Cre/+;TMT and Opn4-Cre/Cre;TMT mice with intact or 1DPI optic nerves, with Tuj1 (red) and pS6 (blue) staining. Arrows point to TMT+ cells expressing pS6. (E) Quantification of the percentages of TMT+ ipRGCs expressing pS6. *P < 0.05, ANOVA followed by Tukey’s test, three to five mice in each group. (F) Whole-mount retinas from WT mice injected with AAV-GFP or AAV-Mela with intact or 1DPI optic nerves, with Tuj1 (red) and pS6 (green) staining. (G) Quantification of the percentage of Tuj1+ RGCs expressing pS6. *P < 0.05, ANOVA followed by Tukey’s test, three to five mice in each group. (Scale bars, A, D, F, 50 μm.)