Fig. 1.
Generation and functional characterization of HSL-containing hydrogels. (A and B) The release profile of HSL (●) and non-HSL (○) on heating was assessed by the quenching properties of calcein. Percent release as a function of temperature (A) and the time course of release at 42 °C (B) were measured (80% within 5 min; 100% within 10 min). (C) HSL-containing hydrogels were observed by TEM (red arrows: HSL; white arrows: phage particles; dark particles: AuNP). (Inset) HSL alone. (Scale bar, 50 nm.) (D) UV-vis spectra at serial phage titers (1–6), starting from 7.5 × 107 TU/μL and diluted 1:4 sequentially to 7.3 × 104 TU/μL. (E and F) Release profiles of HSL- (●) and non–HSL- (○) containing hydrogels as a function of temperature (E) and temporal release profile at 42 °C (F). Shown data are mean ± SEM.