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. 2016 Feb 1;113(7):E932–E941. doi: 10.1073/pnas.1517181113

Fig. 6.

Fig. 6.

Slowing of pacemaker activity of Cav1.3−/− SAN myocytes after ACh inhibition. Histograms of spontaneous action potential rate recorded in control conditions and after perfusion of ACh at the indicated doses in SAN myocytes from WT (A, Top), Cav1.3−/− (B, Top), Cav1.3−/−/Girk4−/− (C, Top), and n = 9 Girk4−/− (D, Top) mice. Examples of spontaneous SAN cells action potentials recorded from WT (A, Bottom), Cav1.3−/− (B, Bottom), Cav1.3−/−/Girk4−/− (C, Bottom), and Girk4−/− (D, Bottom) mice in control conditions (black traces) and after perfusion of ACh (0.05 μM, gray traces). (A–D) Statistics: one-way ANOVA followed by Tukey’s multiple comparisons test. (E and F) Dose–response relation of the percentages of rate reduction at different doses ACh in SAN cells isolated from WT, Cav1.3−/−, Cav1.3−/−/Girk4−/−, and Girk4−/− mice. (E) Statistics: two-way ANOVA followed by Tukey’s multiple comparisons test. The number of SAN cells measured is reported in SI Appendix, Table S7 for each genotype and ACh concentration. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.