Fig. 5.

The transcriptional program of K14⁺ tumor cells is enriched for desmosome and hemidesmosome adhesion complex genes, and depleted for genes involved in MHC class II immunosurveillance. (A) K14⁻ and K14⁺ cells were isolated by FACS from K14-actin-GFP;MMTV-PyMT tumors and the relative mRNA expression of K14 relative to GAPDH was determined by quantitative RT-PCR. The relative mRNA expression is presented as a boxplot (n = 4 independent experiments). (B) Heatmap of the most differentially expressed genes determined by RNA sequencing of K14⁻ and K14⁺ cells at a genome-wide significance of P < 10⁻⁶. (C–C′′) Genes that encode for desmosome, hemidesmosome, and MHC class II antigen presentation protein complexes are differentially expressed and coregulated in K14⁺ cells.