Fig. S6.

K14⁺ cells and K14⁻ cells are enriched for different stemness genes and are not significantly different in their mammosphere forming ability. (A) Relative expression of different stem cell-associated genes presented as a dot-plot. P value determined by Mann–Whitney test. (B) Enrichment in K14⁺ and K14⁻ cells was determined by Wilcoxon gene set testing using published stem cell gene sets identified by transcriptome profiling of mouse mammary gland. (C) Representative micrographs of mammospheres generated from K14⁻ and K14⁺ single cells in suspension culture. (Scale bars, 100 μm.) (D) The median number of mammospheres is presented as a dot plot for K14⁻ and K14⁺ single tumor cells isolated by FACS and plated at two different densities. P value determined by the Mann–Whitney test.