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SDS/PAGE analysis of VinK–ACPs cross-linking products. (A) Cross-linking reaction of VinK with VinP1LdACP in the presence of BMOE. SDS/PAGE after the reaction of VinK with VinP1LdACP for 10 and 60 min is shown. The bands corresponding to the complex formation were apparent from the reaction mixture of the VinK S266C mutant with VinP1LdACP. (B) Purified covalent VinK–VinL (lane 1) and VinK–VinP1LdACP (lane 2) complexes.
