Table 1.

Affinity of VinK to VinL determined by ITC

VinK	VinL	Ka × 103,* M−1	ΔG, kcal/mol	ΔH, kcal/mol*	−TΔS, kcal/mol	n
Wild type	Wild type	82.1 ± 7.7	−6.7	−8.4 ± 0.2	1.7	1.11 ± 0.02*
R153A	Wild type	1.9 ± 0.0	−4.4	−6.3 ± 0.1	1.9	1.0†
M206A	Wild type	6.2 ± 0.2	−5.1	−5.5 ± 0.1	0.4	1.0†
R299A	Wild type	0.8 ± 0.1	−4.0	−5.6 ± 0.3	1.6	1.0†
Wild type	D35A	12.0 ± 0.6	−5.6	−6.7 ± 0.1	1.1	1.0†
Wild type	E47A	10.9 ± 0.5	−5.5	−7.9 ± 0.1	2.4	1.0†

^*Values are ± SD from the fit.

^†n was fixed at 1 for the purposes of fitting the data because the affinity was below the level for accurate determination of binding stoichiometry.