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. 2015 Jul 23;98(6):1027–1035. doi: 10.1189/jlb.2A0515-209R

Figure 1. HIV-1-infected CD4+ T cells internalized AAT through a clathrin-dependent endocytosis process.

Figure 1.

Activated primary CD4+ T cells were infected with HIV-1IIIB and then cultured with the presence or absence of methyl-β-cyclodextrin, filipin or nystatin, or chlorpromazine (all 20 µg/ml) for 1 h. Next, these T cells were incubated with the presence or absence of 5 mg/ml Alexa Fluor 488-labeled AAT. After 3, 12, or 24 h of incubation, the cells were collected, fixed, and imaged with a confocal microscope (×600).