Figure 1.

Notch signaling is hyperactive, and secretory differentiation markers are down-regulated in the setting of Mtgr1 loss. A) Heatmap of dysregulated Notch-associated genes in microarray from laser-captured small intestinal crypts from WT and Mtgr1^−/− mice (17). B) qPCR of the indicated lineage markers in small intestines (left) and colons (right) of WT (n = 4) and Mtgr1^−/− (n = 4) mice. C) Heatmap of secretory-associated transcripts in WT and Mtgr1^−/− MSIE cells. D) Representative images of enteroid cultures from WT and Mtgr1^−/− mice 1, 3, and 5 d after plating. E) Heatmap of Notch-associated genes in WT and Mtgr1^−/− small intestinal-derived enteroids. Red denotes increased fold expression relative to WT, and green denotes decreased fold expression relative to WT. The statistical test used was the Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.