Figure 6.

MTGR1 interacts with transcriptional corepressor GFI1 and can repress GFI1 targets. A) HA:MTGR1 and Flag:GFI1 were transiently expressed in COS7L cells. Flag:GFI1 was immunoprecipitated, and HA:MTGR1 was blotted for in immune complexes (IC). B) GFI1 repression targets Satb1, E2f5, and Jak3 were up-regulated in laser-captured crypts from WT and Mtgr1^−/− mice (17). C) HA:MTGR1 was transfected into NIH 3T3 cells with the transcriptional reporter Satb1-Lux. Luciferase activity was quantified in whole-cell extracts by luminometry. Results were normalized to an internal Renilla constitutive reporter. Ctrl., control. The statistical test used was the Student’s t test. ***P < 0.001.