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. 2015 May 21;29(9):3762–3772. doi: 10.1096/fj.15-271635

Figure 3.

Figure 3.

C5a-induced ALI is dependent on Ly-6G+CD11b+ PMNs (C5a dose: 20 ng/g BW, i.t; negative control: equal dose of PBS). A) CD11b and Ly-6G surface expression on BALF cells was assessed by flow cytometry. Mice were subjected to C5a-ALI for 8 h. B) Top: Ly-6G expression in lungs of C57BL/6J mice that had been subjected to C5a-ALI or PBS sham surgery. Bottom: transmission electron micrographs of lungs 8 h after administration of rmC5a or PBS. Arrowheads: fibrin depositions; arrow: a damaged type II alveolar epithelial cell. Magnification, ×2600. C) Experimental neutropenia was induced by injection of a depleting antibody against Ly-6G (α-Ly-6G). Control mice received isotype IgG (Ctrl IgG). To assess neutropenia, automated complete blood counts were acquired from EDTA blood. D) Neutropenic mice and Ctrl IgG were subjected to C5a-ALI. Alveolar albumin concentrations and WBCs were detected 8 h after instillation of rmC5a. All images are of C57BL/6J mice. **P < 0.01, ***P < 0.001.