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CCR5 mediates adverse events during C5a-ALI. A) Casein-elicited peritoneal PMNs were isolated, and Ly-6G+CD11b+ cells were analyzed for surface expression of CCR5 by flow cytometry. Isotype antibody was used as the control. Data are representative of 3 independent experiments. B) BALF albumin concentrations and WBC counts 8 h after administration of rmC5a (20 ng/g BW, i.t.) in C57BL/6J mice or CCR5−/− mice. *P < 0.05, **P < 0.01.
