Fig. 4. Mechanistic studies and resistance to proteases. (A) Flow cytometry data describing pharmacological inhibition of dynamin-mediated endocytosis by dynasore,23 membrane fluidity by methyl-β-cyclodextrin24 (M-βCD) or membrane trafficking by a reduction in incubation temperature. Data is normalized to DPBS at a value of 1. (B) Proteolytic susceptibility was determined by comparing RP-HPLC chromatograms of the material before and after treatment with trypsin or the protease cocktail pronase. Standard curves and individual chromatograms are provided in Fig. S22–S25.† .
