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. 2016 Feb 22;11(2):e0149926. doi: 10.1371/journal.pone.0149926

Fig 4. AMD3100 increased inflammatory cytokine secretion, and promoted CD3-positive cell infiltration following UUO.

Fig 4

A. Expression of renal inflammatory cytokines (IL-6, TNF-α, IL-10 and IFN-γ) was detected by RT-PCR. B. Percentages of different inflammatory cell types (cells positive for CD45, CD3, CD11b and F4/80) in the kidney were measured by flow cytometry. C. Immunofluorescence was used to further determine CD3-positive cell infiltration into the kidney, and the number of infiltrated CD3-positive cells was determined. (***p<0.001, **p<0.005, *p<0.05, n.s. means no significant difference, scale = 100μm, magnification×400)